Would the Great Race of Yith, the Elder Things, and Lavinia Whateley from the Cthulhu Mythos be okay? The first two are neuter and the last one is a cis woman, after all. (Yes, I realize the irony in nominating that canon.)

My other planned nominations are less confusing.

Atlan (obscure early New Weird series about a princess in a nightmarish Atlantis)
Cija (cis woman)
Lel (trans woman)
Seka (cis girl)


Jirel of Joiry (short story cycle about a warrior woman's adventures)
Jirel of Joiry (cis woman)

Star Control II ('92 DOS space opera game full of unforgettable characters and artwork)
Talana (cis woman)
Queen Brakky Girdy (cis...female)